UCSF CCMBM Cores: Featured Services

UCSF CCMBM Core Services - November 2018

CCMBM Cores:
Featured Services

The Core Center for Musculoskeletal Biology & Medicine (CCMBM) defines itself by emphasizing musculoskeletal-specific events, grants, and services that are critical to its members but not available elsewhere in the university system.

“As a CCMBM Member, I was able to apply and received a core voucher grant that was used for micro-ct imaging of samples. Access to micro-ct scanning provided a high throughput and rigorous analysis of my samples. Upon receiving the grant, data was generated quickly and was able to be included in a recent grant application. Full analysis of the samples will certainly be included in upcoming publications and presentations. Without the voucher, significant funding and time would be have been diverted away from other key resources required for my research.”
– Daniel Clark, DDS, MS, Postdoctoral Scholar, Department of Orofacial Sciences

ACKNOWLEDGING THE CCMBM

If you have received a CCMBM grant, formed a collaboration at one of our events, or used one of our cores, please acknowledge the CCMBM in related publications and presentations. We suggest stating:

Research reported in this publication was supported by the UCSF Core Center for Musculoskeletal Biology and Medicine (CCMBM) of the National Institute of Health's National Institute of Arthritis and Musculoskeletal and Skin Diseases (NIAMS) under the award number P30AR066262 .

UPCOMING CCMBM EVENTS & OPPORTUNITIES

November 6, 12:00-1:00pm; Mission Bay, MH-2100
CCMBM EBSD Core Seminar - Beyond Sarcopenia
Stephen B. Kritchevsky, PhD, Sticht Center for Healthy Aging and Alzheimer’s Prevention, Wake Forest School of Medicine.
RSVP to Lucy Wu. Lunch provided with registration.
-----
Specific Aims Review
Have your specific aims page reviewed by established investigators as well as peers.

Reviews are available for CCMBM members at the following locations:
1. General sign up (Mission Bay)
2. Ortho-related sign up (Parnassus)
3. Radiology-related sign up (China Basin)

Become a CCMBM member to be eligible for these aims review.

CCMBM Core Service Voucher Program
Application Deadline – November 30, 2018, 5:00 PM (PST)

The CCMBM Core Voucher Program is designated to enhance utilization of the CCMBM Cores, especially among CCMBM Members who are Early Stage Investigators, and/or New Users.

CCMBM Service Vouchers can be used for direct application of the CCMBM Core that best suits your research needs for up to $5,000, funding the following:
· Pilot work to generate preliminary data
· Development of a new test or service
· Chemicals and supplies to support a designated project
· Fee-for-service work
· Novel, currently unfunded use of a core for an ongoing project

Applications are currently being accepted. Must be a CCMBM member to eligible. Please see the CCMBM funding page for more details.

IMAGING CORE

The Imaging Core has a wide range of muscle imaging and muscle analysis tools available to CCMBM members with a particular focus on muscle fat infiltration and muscle volume or area. For accurate fat quantification, novel, high-resolution chemical shift based MR imaging methods are used. These methods provide fat fraction maps where each voxel represents the exact fat content in that voxel and thus allow characterization of the spatial distribution of intermuscular adipose tissue (IMAT), including differentiating between fat within the muscle regions (intraMF) and between the muscle regions (interMF). Several MR imaging methods can be used to enhance contrast of muscles in order to segment the muscle regions and determine volume or area. For more information, please contact Roland Krug, PhD, CCMBM Imaging Core Co-Director.

Thigh Muscle: Individual muscle regions of interest for quadriceps, hamstrings, and other muscle groups can be manually segmented on axial T1-weighted images. The intraMF fraction, intraMF area and lean anatomical cross sectional area (ACSA) can be calculated for the individual muscles compartments and for all muscle compartments. Lean ACSA for each muscle is the area of the muscle minus the area of the intraMF. The SAT and IMAT regions can also be segmented using an automatic algorithm.

Fat Fraction Maps of the Thigh Muscle Compartments.
Shown are the fat fraction maps (left with color bar units in % fat), color-coded fat fraction maps highlighting the intraMF fraction (red), intermuscular fat fraction (green) and SAT (blue) regions. Also shown are the compartments with the quadriceps (red), hamstrings (green) and other muscle (blue) regions. (Kumar et al. 2014)

Paraspinal Muscle: Volume and mean fat-fraction can be gained from quantitative MRI of the paraspinal muscles. Measurements of manually-defined paraspinal muscles, multifidus (MF) and erector spinae can be reliability obtained. Furthermore, SAT cross-sectional area can be easily segmented from the fat fraction maps by enlarging the field of view. In addition, visceral adipose tissue (VAT) and total adipose tissue (TAT) can also be gained from these MR images.

Fat Fraction Map of the Paraspinal Muscles

Shoulder Muscle: Studies have demonstrated that fatty infiltration of the Rotator Cuff (RC) musculature is one of the critical factors for the clinical outcome after RC repairs. Four muscle groups can be identified and manually segmented as shown in the figure. Again, volume as well as fat content can be determined by quantitative MRI for each muscle compartment.

Shoulder Muscles

ACKNOWLEDGING THE CCMBM