Produce GRAM QUANTITIES
of Recombinant Proteins Expressed in Mammalian Cells in a Hollow Fiber Bioreactor
A hollow fiber bioreactor from FiberCell Systems is the ideal method for producing large quantities of recombinant proteins and other secreted products from mammalian cells. A hollow fiber bioreactor has unique advantages versus other cell culture methods:
Cells are cultured at high density, 1-2 10x108 cells per mL, tissue like density, so large numbers of cells can be cultured in a compact space.
Cells are bound to a porous support, not a non-porous plastic dish so cells do not require passaging and cultures can be maintained on a continuous basis for up to many months or even a year.
The hollow fibers are like filters with a molecular weight cut-off of 5 kD or 20 kD, so products secreted by the cells can be concentrated up to 100 times compared to regular cell culture methods.
A hollow fiber bioreactor can support from 1x109 to 5x1010 cells in a standard incubator. Protein concentrations will be between 100 ug/mL to 300 ug/mL in a harvest volume of 20 mL or less. Harvesting is performed every days. Hollow fiber bioreactors can facilitate the use of a chemically defined medium such as FiberCell Systems' CDM-HD. Post-translational modifications are uniform and complete and the expression of “difficult-to-express” proteins is facilitated. Easy cell culture scale-up with 100 X greater productivity is available for any lab.
Article: Hollow Fiber Bioreactors and Difficult to Express Proteins
Hollow fiber bioreactors provide a unique cell culture environment that results in more in vivo-like cellular physiology and complete and uniform post-translational modifications for complex proteins. A recent article we wrote serves as a follow-up to the webinar and is titled, “Hollow Fiber Bioreactors and Difficult-to-Express Proteins.”
A shorter version was recently published in GEN, the article can be seen here: MORE >>
Webinar: Beyond 3-D: Recapitulating Nature for Optimal Bioproduction
FiberCell Systems, in conjunction with The Scientist, presented a webinar; “Beyond 3-D: Recapitulating Nature for Optimal Bioproduction.” It’s now available on our website for viewing at you convenience.
Difficult-to-express proteins can be proteins that are expressed at low titers by mammalian expression systems. They are also defined as highly complex proteins; highly glycosylated, with high levels of post-translational modifications and in some cases consisting of several subunits. Case studies using hollow fiber bioreactors (HFBR) have demonstrated superior expression of these types of proteins with complete and uniform post-translational modifications, such as hexerimized IgG and the IL15 ligand-receptor complex. The next generation of therapeutic proteins include bi-specific and tri-specific antibodies, protein structures that are the creation of bio-engineers and not found in the templates of nature. These proteins require the in vivo-like cell culture conditions and concentration that HFBRs and mammalian expression systems for proper expression.
A Closer Look at the FiberCell Systems Cartridge
Take a look at our latest infographic explaining the advantages of using hollow fiber bioreactors for cell culture.
Hollow Fiber Cell Culture for Monoclonal Antibodies and Recombinant Proteins
A hollow fiber bioreactor is the ideal method for producing up to gram amounts of antibody or recombinant proteins using a protein-free medium, and at high concentrations.
Hollow Fiber Cell Culture is the Original 3-D Culture System
Hollow fiber cell culture is the original 3-D culture system and is demonstrated to be the most in-vivo-like way of growing cells. We are continuing to find new applications in which a hollow fiber system can recapitulate the in vivo environment. It is an ideal method for cell co-cultivation and is used to culture cryptosporidium in an in vitro gut model. We have also worked with an organization that is using the hollow fiber system to culture and produce large quantities of trypanosomes.
The CD we will send to you includes the complete FiberCell Systems User Manual, instructional video clips, our bibliography and more. There is no obligation.
A hollow fiber bioreactor from FiberCell Systems is the ideal method for producing large quantities of recombinant proteins and other secreted products from mammalian cells. A hollow fiber bioreactor has unique advantages versus other cell culture methods:
