Emerging methods for exosome purification and process monitoring

One of the handicaps of working with bacteriophages is the long duration required to perform plaque assays. Plaque assays also impose questions about accuracy and
precision relative to the scale and experience of the persons performing and interpreting them. This poster presents a pair of high precision, high accuracy chromatography-based assays that permit determination of phage concentration in less than 1 hour.

This poster was presented at Bacteriophage Therapy Summit in March.

Serotype 10 adeno-associated virus (AAVrh_10mCherry) was analysed on the PATfix™ HPLC system with the  CIMac™ AAV full/empty analytical column to estimate the ratio of empty and full AAV particles based on the peak area of the chromatogram given with three different detectors. AAV included a protein capsid containing single stranded DNA. CIMac™ AAV column consisted of a strong anion exchanger with QA chemistry (quaternary amine).

Poster was prepared by Blaž Goričar and presented at ISBioTech 9th Spring Meeting where it was awarded the first prize. Congratulations!

 

In bacteriophage research and therapy, most applications ask for highly purified phage suspensions as such it is crucial to reduce proteins, endotoxins, DNA and other contaminants. In this study we present efficient two-step chromatographic purification method with binding phages to a stationary phase - monoliths. The aim of the study was to develop robust, fast and effective virus purification platform that can be used for several types of bacteriophages for any application.

This poster was presented at Bacteriophage Therapy Summit in March.

The Cell & Gene Meeting on the Mediterranean is a three-day conference bringing together the entire cell and gene therapy community from Europe and beyond. Between April 23 and 25, this program will cover a wide range of commercialization topics from market access and regulatory issues to manufacturing and financing, featuring expert-led panels, extensive one-on-one partnering capabilities, exclusive networking opportunities, and 50+ dedicated presentations.

Meet us there!
You are most welcome to visit our booth and let our team present you all the advantages of monolithic technology.