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Volume 2 Issue 2, February 2021

The Director’s Corner

Carrie Harmon – Executive Director, NPDN
 
Dear NPDN friends,
 
I am humbled to see each month the results of the work you do to serve your local diagnostic clientele, and the incredible work being done through the NPDN committees to benefit the entire network. To keep on top of the many projects in NPDN, remember to keep in touch with your region’s NPDN folks, check out the newly revised NPDN portal, especially the announcements and committee pages, and read up on highlights in this newsletter.

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Protocols and Validation Quick and Useful Tips

Tissue Homogenization for Nucleic Acid Extraction Tips

Samantha Fieweger, Wisconsin Department of Agriculture, Trade and Consumer Protection, and Laura Miles, Michigan State University
In plant diagnostic laboratories that routinely run molecular tests, the efficient disruption and homogenization of plant tissues is a critical step for reliable and reproducible detection of plant pathogens. The tissue homogenization technique is coupled to a nucleic acid extraction protocol, and together they will determine both the quantity and quality of the DNA or RNA used in downstream applications.
Choosing the correct tissue homogenization technique not only depends on the type of plant material, but also on the available budget and equipment. There are many different homogenization techniques, and a few that we use in our labs are discussed below.
Prior to using any of the described techniques, hard plant tissue can also be ground in liquid nitrogen or freeze-dried (although a lyophilizer is an expensive piece of equipment) to obtain a fine powder. These pretreatments are effective alternatives to ensure good tissue maceration.

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NPDN Lab Interview with Dr. Raj Singh

LSU AgCenter Plant Diagnostic Center (SPDN)

Raj, give us a little history of your lab.

Raj SinghThe lab was established as a plant diagnostic clinic in 1972 and was operated mostly by plant pathology faculty with Extension appointments until 2007. I was hired in 2007 as a full-time diagnostician to run the clinic. In 2010, the clinic was transformed into a state-of-the-art Plant Diagnostic Center with a goal of providing statewide diagnostic programs for all plant health problems. The current Plant Diagnostic Center provides “one stop shopping” for the entire state of Louisiana, accepting any type of plant health problem from commercial or residential clients, as well as LSU AgCenter Extension personnel. We are working toward STAR-D accreditation.

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Announcements

  • APS 2021 Spring Webinars

  • VALITEST Webinar Series and Training Activities Update

  • GPDN 2021 Webinar Series

Read Announcements

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