Welcome to the Winter 2021 FiberCell Systems Expressions Newsletter
FiberMan came out of the lab and saw his shadow so there will unfortunately be six more weeks of winter. FiberMan has been wearing his mask, practicing social distancing, and washing his hands very often. We are looking forward to the time when we can all meet up again. The days are already getting longer and though there is snow on the ground here in FiberLand it feels like spring, and a rebirth of all things is just around the corner.
SBIR Grant Awarded
 FiberCell Laboratories, along with its partner FiberCell Bio-Productions, was recently awarded a Phase I SBIR Grant for an investigational study to produce engineered extracellular vesicles from mesenchymal stem cells for the treatment of deep wounds and chronic ulcers. One drawback associated with EV tissue regeneration can be the formation of scar tissue, painful and unsightly. FiberCell Laboratories will evaluate a proprietary molecule to enhance wound healing while reducing scar tissue formation in a topically applied formulation. Hollow fiber bioreactors are a viable method for the production of clinical scale and clinical grade extracellular vesicles for various therapies.
 Click to learn more EV production with hollow fiber bioreactors →
Monoclonal Antibody Case Study
It’s always fun to guide a new user through their first monoclonal antibody production run in a hollow fiber bioreactor. It’s even more fun when the results exceed everyone’s expectations, even ours! And what makes these results extra special, is that the researcher needed to work without antibiotics and had issues with contamination. The harvest volume is large because of extra harvests due to the addition of insulin, which was later removed.
| Cartridge |
C2011 |
| Cell Line |
SP2 Mouse hybridoma |
| Medium |
Inoculation medium: DMEM/10% FBS (insulin supplement, then removed), switched to production medium: DMEM/10% CDM-HD |
| Medium consumed |
37 liters total |
| Harvest volume |
1200 mL |
| Antibody produced |
1.9 grams |
| Total time |
49 days |
| Average concentration (After purification) |
1.59 mg/mL |
Click for more information on insulin, CDM-HD and hybridoma culture →
Hot off the Presses!
 New Article: Hollow-fiber bioreactor production of extracellular vesicles from human bone marrow mesenchymal stromal cells yields nanovesicles that mirrors the immune-modulatory antigenic signature of the producer cell.
This new article demonstrates the potential for FiberCell System’s 3-D artificial capillary hollow fiber bioreactor to produce clinical grade and clinically relevant amounts of extracellular vesicles from bone marrow mesenchymal stem cells, and likely from other sources as well. The module used here is our C2011 MEDIUM sized bioreactors, with 4,000 cm2 of surface area and 20 mL of extracapillary space. FiberCell Systems also has available as a standard product the C2018 cartridge with 1.2 m2 of area and a 70 mL extracapillary space volume. One estimate is that 6 of the C2018 could produce gram quantities of EVs in a month’s time.
Click to read full article →
 Mini-webinars
If you are like me, you get lots of invitations to view lots of different webinars, but you find it difficult to devote an entire hour-- even if the topic is of great interest. FiberCell Systems has put together a series of 4 shorter “mini-webinars”. They won’t take up too much of your time!
Tech tip: Antibiotics and Cell Culture
 The hybridoma case study presented in this newsletter, shows the importance of good sterile technique when working with the cartridges. FiberCell Systems generally recommends the use of antibiotics as a safety measure, but there are occasions when you do not want to work with antibiotics. For cultures that require very low endotoxin levels you should not work with antibiotics. Low levels of bacterial infections can occur and be hard to detect, but will secrete endotoxin into the media. Working antibiotic-free will ensure endotoxin free cultures, but at the risk of contamination. It is okay to use pen/strep in a media solution but once a month, take a sample from the extracapillary space and reservoir bottle and place in a small flask with antibiotic-free medium to make sure you have a clean culture. Also, make sure you do not freeze your cells down with antibiotics. That way the first week of bringing them out of cryo-storage can serve as a sterility check.
Click for more tech tips →
Thanks for your time, and please stay safe.
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