 Welcome to the Spring 2021 FiberCell Systems Expressions Newsletter
DiFi cells cultured in our C2011
We always love to get interesting photos and data from our end users. This is a cut-open cartridge sent to us by Dr. Jeffrey Franklin at Vanderbilt University. These are DiFi cells (human colorectal cancer cell line) cultured in our C2011 (20 kd MWCO) cartridge with DMEM/10% CDM-HD (our protein-free serum replacement). Supernatant EVs were collected on a daily basis from this cartridge, that was seeded August 14, 2020, until April 8, 2021.
In general, protein-free CDM-HD helps to control the cell mass in the cartridge as the cells are not as adherent to one another. In this case, after 8 months the cartridge became so densely packed with cells that very little harvest volume could be collected. The culture was terminated and cut open to observe the cells. It is easy to see how densely packed the cells are. A fiber bundle sample was also placed in formalin for histology. The literature on histology and microscopy of hollow fiber cultures is a little sparse, so if anybody has experience with thin section, cryosection, immunolabeling or electron microscopy of hollow fiber bioreactors in situ we would love to hear from you! (Thanks Dr. Franklin!)
 
The Evidence is Growing!
Several papers have come out recently demonstrating that not only is a 3-D hollow fiber bioreactor capable of producing extracellular vesicles in clinically relevant quantities in a potentially cGMP compliant system, these extracellular vesicles are showing enhanced bioactivity. This is not surprising given the more in vivo-like cell culture conditions provided by our hollow fiber bioreactors.
Three-dimensional culture of MSCs produces exosomes with improved yield and enhanced therapeutic efficacy for cisplatin-induced acute kidney injury. Cao et al. Stem Cell Research & Therapy (2020) 11:206
 Read the article →
Hollow-fiber bioreactor production of extracellular vesicles from human bone marrow mesenchymal stromal cells yields nanovesicles that mirrors the immunomodulatory antigenic signature of the producer cell. Gobin et al. Stem Cell Research & Therapy (2021) 12:127
Read the article →
Amniotic stem cell EVs produced in a 3-D hollow fiber bioreactor have enhanced bioactivity when compared to 2-D petri dish EVs, with yields that can support clinical applications in a cGMP compatible system. L. Perin et. al. ISEV 2020 poster.
View the poster →
3-D hollow fiber bioreactors permit the recapitulation of some complex biological systems
I worked directly with Dr. Barbara Ballerman and Dr. Paul Cahill as they developed a method for the culture of endothelial cells under shear stress on the insides of the fibers. This was nearly 30 years ago! I always thought that there should be an application for modeling diabetic retinopathy but it seemed to be a great challenge. I was personally gratified to see the following publication recently, that also features co-cultivation of retinal endothelial cells with mouse peripheral mononuclear cells and the effects of elevated glucose concentration. It includes a very nice 3-D model showing cell to cell interactions in a hollow fiber bioreactor.
Application of a Flow-Based Hollow-Fiber Co-Culture System to Study Cellular Influences under Hyperglycemic Conditions, Ebrahim et.al. 07 March, 2019
Read the article →
Now out!
The Hybridoma Troubleshooting Guide has just been published and is available for download on the FiberCell Systems website. We collected all of the helpful tips and tricks we have learned over the years, in conjunction with our end users and pulled them all together into one, easy to use resource.
One common problem in hollow fiber hybridoma cultures is the overgrowth of cells, the cell numbers become too high for the system to deliver oxygen, resulting in anaerobic metabolism.
Hope to see you all at some meetings this Fall!
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Request a FREE Hollow Fiber Instruction Manual
The USB we will send to you includes the complete FiberCell Systems User Manual, instructional video clips, our bibliography and more.
There is no obligation.
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